Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: Raman spectra acquired from cytoplasmic (A) and nuclear (B) regions of the same live HeLa cell that was incubated at 37°C for 60 h in a CoMoCAT DM-SWNT dispersion. The colored arrows in the optical micrographs denote the specific regions of the HeLa cell where spectra were acquired; spectra were also acquired from cell-free regions of the culture dish ~5 μm away from the nearest cell (dark-blue arrows). All spectra were normalized to the same intensity scale.

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Incubation, Dispersion

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: Raman spectra acquired from live HeLa cells incubated at 4°C in a CoMoCAT DM-SWNT dispersion; both spectra were normalized to the same intensity scale as that in Figure 5.

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Incubation, Dispersion

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: Representative Raman spectra acquired from five different live HeLa cells that were incubated at 37°C in CoMoCAT DM-SWNT dispersions for 12, 24, 36, 48, and 60 h. All spectra were normalized to the same intensity scale. The G-band intensities increased in a linear fashion (R 2 = 0.932) over the course of 12–60 h (n = 8 cells analyzed at each time point).

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Incubation

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: TEM micrographs of control HeLa cells that were incubated for 60 h at 37°C in DMEM/FBS (no DM-SWNTs). All slices were treated with uranyl acetate to stain membranes and lead citrate to stain the nuclear body. Colored arrows represent selected cell organelles: nuclei (red), mitochondria (green), Golgi bodies (yellow), vacuoles (blue), and the nucleolus (pink). Micrographs were normalized to the same grayscale as those in Figure 9.

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Control, Incubation, Staining

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: TEM micrographs of HeLa cells that were incubated for 60 h at 37°C in CoMoCAT DM-SWNTs. All slices were treated with uranyl acetate to stain membranes and lead citrate to stain the nuclear body. Colored arrows represent selected cell organelles: nuclei (red), mitochondria (green), Golgi bodies (yellow), and vacuoles (blue). Micrographs were normalized to the same grayscale as those in Figure 8.

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Incubation, Staining

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: Representative differential image contrast (DIC) images of live HeLa cells incubated for 60 h at 37°C in DMEM/FBS (A) or CoMoCAT DM-SWNTs (B) .

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Incubation

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: Growth curves for living HeLa cells incubated at 37°C for 4 d in DMEM/FBS or DM-SWNTs. The final concentration of SWNTs in DMEM/FBS was estimated to be ~50 μg/mL (Additional File ) and SWNT lengths were estimated to be 100–400 nm (Additional File ).

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Incubation, Concentration Assay

Journal: Journal of Nanobiotechnology

Article Title: Single-walled carbon nanotube interactions with HeLa cells

doi: 10.1186/1477-3155-5-8

Figure Lengend Snippet: Flow cytometry analysis of intracellular MitoSOX™ Red fluorescence from live HeLa cells incubated at 37°C for 60 h in: (A) DMEM/FBS, (B) CoMoCAT DM-SWNTs, (C) DMEM/FBS + MitoSOX™ Red, (D) DM-SWNTs + MitoSOX™ Red, and (E) DMEM/FBS + MitoSOX™ Red + H 2 O 2 . The x -axis denotes the MitoSOX™ Red fluorescence detected in the 564–606 nm spectral region and the y -axis denotes the number of events recorded for each analysis.

Article Snippet: Human epithelial-like HeLa cells were obtained from the American Type Culture Collection and were cultured in 100 mm diameter polystyrene tissue culture dishes (Sarstedt) in DMEM/FBS containing 15 mg/L phenol red in an incubator at 37°C with 90% air and 10% CO 2 .

Techniques: Flow Cytometry, Fluorescence, Incubation

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Changes in the physiological state of A549 and iMSC cells after 24 h of incubation with sodium citrate, S. fallax moss extract, and citrate-stabilized (AgNP-cit) and extract-stabilized (AgNP-sf) silver nanoparticles at concentrations of 1, 6, and 12 µg/mL. MTT test ( A ); resazurin reduction assay ( B ). ns: insignificant, * p < 0.05, ** p < 0.01.

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Incubation

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Microimages of A549 and iMSC cells treated with trypan blue and the results of the analysis with propidium iodide (in the form of a table located in the upper right corner) to determine the necrotic effect. The cells were incubated for 24 h with sodium citrate, S. fallax moss extract, and citrate-stabilized (AgNP-cit) and extract-stabilized (AgNP-sf) silver nanoparticles at concentrations of 1, 6, and 12 µg/mL. Arrows denote altered cells.

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Incubation

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Analysis of the cell confluence.

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Control

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Real-time monitoring for 80 h of the cell index of A549 and iMSC cells incubated with sodium citrate, S. fallax moss extract, and citrate-stabilized (AgNP-cit) and extract-stabilized (AgNP-sf) silver nanoparticles at concentrations of 1, 6, and 12 µg/mL. The time of adding nanomaterials is indicated by an arrow (24 h).

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Incubation

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Dark-field images ( A , C – E , I – K ) and hyperspectral analysis ( B , F – H , L – N ) of A549 control cells ( A , B ) and A549 cells incubated with extract-( C – H ) and citrate-( I – N ) stabilized AgNPs at different concentrations (1 µg/mL ( C , F , I , L ), 6 µg/mL ( D , G , J , M ), and 12 µg/mL ( E , H , K , N )) for 24 h.

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Control, Incubation

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Visualization of the formation of multicellular spheroids from cells treated with silver nanoparticles using optical microscopy: control ( A , H ); spheroids from A549 cells incubated with extract-stabilized silver nanoparticles at concentrations of 1, 6, and 12 μg/mL ( B , C , and D , respectively); spheroids from A549 cells incubated with citrate-stabilized silver nanoparticles at concentrations of 1, 6, and 12 μg/mL ( E , F , and G , respectively); spheroids from iMSC cells incubated with extract-stabilized silver nanoparticles at concentrations of 1, 6, and 12 μg/mL ( I , J , and K , respectively); spheroids from iMSC cells incubated with citrate-stabilized silver nanoparticles at concentrations of 1, 6, and 12 μg/mL ( L , M , and N , respectively).

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Microscopy, Control, Incubation

Journal: Biomolecules

Article Title: Advances in the Toxicity Assessment of Silver Nanoparticles Derived from a Sphagnum fallax Extract for Monolayers and Spheroids

doi: 10.3390/biom14060611

Figure Lengend Snippet: Visualization of spheroids from A549 control cells ( A , B ), after incubation with extract-stabilized ( C – H ) and citrate-stabilized ( I – N ) silver nanoparticles for 24 h.

Article Snippet: The study used epithelial-like human lung carcinoma cells (A549) (ATCC, Manassas, VA, USA) and a genetically stable immortalized MSC line derived from adipose tissue (iMSC).

Techniques: Control, Incubation